Stereology offer morphometric data is helpful in TPN-AC study.ДЄbstract = 'Total parenteral nutrition has been used in surgical field. Branched chain amino acid enriched solution is a better solution in TPN. With wider bile canaliculi pathways and more efficient mocrovilli in Gr.III, total parenteral nutrition associated cholestasis is less prominent in Gr.III. From the above data, we concluded that bile canaliculi become expanded and rounded in Gr.III, the microvilli became elliptical and flattened, which is more efficient in driving the bile. The microvilli changed its surface density in Gr.III, which is significantly increased than the control group. The surface density of bile canaliculi wall in Gr.III is 40% less than the control group, the volume density of bile canaliculi space remained unchanged in all four groups. Using the stereology technique, the relative length, surface, volume of bile canaliculi and microvilli were calculated. Microvilli become edematous, distorted and decreased in numbers in all TPN groups, most severe in Gr.II. After seven days of TPN treatment, the rats were sacrificed, liver tissue harvested for electron microscopic examination and stereology morphometric study.

The main difference between Molipron FВ® and AminicВ® is the concentration of Branched Chain Amino Acid (BCAA) which is 22.6% (Molipron FВ®) versus 35.9% (AminicВ®). Gr.II 20% glucose and 3.5% Molipron FВ®, Gr.III 20% glucose and 3.5% AminicВ®, Gr.IV 30% glucose and 3.5% AminicВ®. Gr.II (N=6) Gr.III (N=7) and Gr.IV (N=6) were treated with intravenous infusion of TPN with different compositions. Gr.I (N=6) was sham operated and fed with regular rat chow. Twenty-five adult rats were divided randomly into four groups. In the present study, we use electron microscopy and stereology to evaluate the effects of different composition of TPN on the morphological changes of hepatic organelles, try to improve the knowledge of total parenteral nutrition associated cholestasis (TPN-AC). One of the side effects is hepatic impairment. Total parenteral nutrition has been used in surgical field.

Stereology basics